验证数据展示
经过测试的应用
| Positive WB detected in | rat pancreas tissue, pig pancreas tissue, pig stomach tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66471-1-Ig targets TFF2 in WB, ELISA applications and shows reactivity with human, pig, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, pig, rat |
| 免疫原 | TFF2 fusion protein Ag18765 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | trefoil factor 2 |
| 别名 | SML1, SP, Spasmolysin, Spasmolytic polypeptide, TFF2, trefoil factor 2 |
| 计算分子量 | 129 aa, 14 kDa |
| 观测分子量 | 16 kDa |
| GenBank蛋白编号 | BC032820 |
| 基因名称 | TFF2 |
| Gene ID (NCBI) | 7032 |
| RRID | AB_2881837 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TFF2 antibody 66471-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
