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验证数据展示

  • FC experiment of HeLa using CL488-66471

    FC experiment of HeLa using CL488-66471

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TFF2 (CL488-66471, Clone:2D7B1) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated TFF2 Monoclonal antibody

TFF2 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66471

产品说明书

CloneNo. 2D7B1

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC (Intra)

别名

SML1, 2D7B1, trefoil factor 2, Spasmolytic polypeptide, Spasmolysin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66471-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66471 targets TFF2 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 TFF2 fusion protein Ag18765 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 trefoil factor 2
别名 SML1, 2D7B1, trefoil factor 2, Spasmolytic polypeptide, Spasmolysin
计算分子量 129 aa, 14 kDa
观测分子量 16 kDa
GenBank蛋白编号BC032820
基因名称 TFF2
Gene ID (NCBI) 7032
RRIDAB_2934464
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ03403
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TFF2 antibody CL488-66471Download protocol
Standard Protocols
Click here to view our Standard Protocols