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TFPI Recombinant antibody

TFPI Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240977B1

Cat no : 83838-5-RR

Print datasheet

Synonyms

TFI, Lipoprotein-associated coagulation inhibitor, LACI, Extrinsic pathway inhibitor, EPI

验证数据展示

  • WB analysis of HepG2 using 83838-5-RR

    WB analysis of HepG2 using 83838-5-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83838-5-RR (TFPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83838-5-RR

    FC experiment of HepG2 using 83838-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TFPI Recombinant antibody (83838-5-RR, Clone:240977B1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HUVEC using 83838-5-RR

    FC experiment of HUVEC using 83838-5-RR

    1x10^6 HUVEC cells were surface stained with 0.25 ug TFPI Recombinant antibody (83838-5-RR, Clone:240977B1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

  • IF Staining of HepG2 using 83838-5-RR

    IF Staining of HepG2 using 83838-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TFPI antibody (83838-5-RR, Clone: 240977B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83838-5-RR

    Affinity and Kinetic Characterization of 83838-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83838-5-RR against Human TFPI were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83838-5-RR is TFPI Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
Synonyms TFI, Lipoprotein-associated coagulation inhibitor, LACI, Extrinsic pathway inhibitor, EPI
Calculated Molecular Weight35 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberNM_006287.6
Gene Symbol TFPI
Gene ID (NCBI) 7035
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10646-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tissue factor pathway inhibitor (TFPI) is a critical anticoagulant protein present in endothelium and platelets. TFPI is produced as two major isoforms in humans, TFPIa and TFPIb that result from alternative splicing. The TFPIb isoform localizes to the endothelium surface where it is a potent inhibitor of tissue factor-factor VIIa complexes that initiate blood coagulation. The TFPIa isoform is present in platelets. TFPI is a Kunitz-type serine protease inhibitor that exerts anticoagulant activity by blocking early procoagulant stimulia. TFPI can enhance anti-thrombotic treatment in sepsis, inflammatory diseases, and cardiovascular diseases.

实验方案

Product Specific Protocols
WB protocol for TFPI antibody 83838-5-RRDownload protocol
IF protocol for TFPI antibody 83838-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols