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验证数据展示

  • IF Staining of HepG2 using CL488-66842

    IF Staining of HepG2 using CL488-66842

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 TFPI antibody (CL488-66842, Clone: 1B12F11 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-66842

    FC experiment of HepG2 using CL488-66842

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TFPI (CL488-66842, Clone:1B12F11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated TFPI Monoclonal antibody

TFPI Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66842

产品说明书

CloneNo. 1B12F11

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

TFI, Lipoprotein-associated coagulation inhibitor, LACI, Extrinsic pathway inhibitor, EPI

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66842-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66842 targets TFPI in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 TFPI fusion protein Ag14980 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
别名 TFI, Lipoprotein-associated coagulation inhibitor, LACI, Extrinsic pathway inhibitor, EPI
计算分子量251aa,29 kDa; 304aa,35 kDa
观测分子量 35-38 kDa
GenBank蛋白编号BC015514
基因名称 TFPI
Gene ID (NCBI) 7035
RRIDAB_2934500
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP10646
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tissue factor pathway inhibitor (TFPI) is a critical anticoagulant protein present in endothelium and platelets. TFPI is produced as two major isoforms in humans, TFPIa and TFPIb that result from alternative splicing. The TFPIb isoform localizes to the endothelium surface where it is a potent inhibitor of tissue factor-factor VIIa complexes that initiate blood coagulation. The TFPIa isoform is present in platelets. TFPI is a Kunitz-type serine protease inhibitor that exerts anticoagulant activity by blocking early procoagulant stimulia. TFPI can enhance anti-thrombotic treatment in sepsis, inflammatory diseases, and cardiovascular diseases.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TFPI antibody CL488-66842Download protocol
FC protocol for CL Plus 488 TFPI antibody CL488-66842Download protocol
Standard Protocols
Click here to view our Standard Protocols