- Featured Product
- KD/KO Validated
TGF Beta 1 Polyclonal antibody
TGF Beta 1 Polyclonal Antibody for FC, IF/ICC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
FC, IF/ICC, IP, WB, ELISA and More (3)
Conjugate
Unconjugated
544
验证数据展示
经过测试的应用
| Positive WB detected in | mouse skin tissue, K-562 cells, HeLa cells, A549 cells, rat skin tissue |
| Positive IP detected in | mouse skin tissue |
| Positive IF detected in | HEK-293 cells |
| Positive FC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy TGF beta 1 Ready-To-Use IHC Kit. TGF beta 1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21898-1-AP targets TGF Beta 1 in WB, IP, IF, FC, IHC, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IF/ICC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC, CoIP, ELISA, Cell treatment |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rabbit, zebrafish, pig, canine |
| Immunogen | TGF Beta 1 fusion protein Ag13591 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | transforming growth factor, beta 1 |
| Synonyms | CED, DPD1, LAP, Mouse Transforming Growth Factor β1, TGF beta 1, tgf beta1, TGFB, TGFB1, TGFbeta, TGF-beta 1, tgfbeta1, tgf-beta1, TGFβ, TGF-β, TGFβ1, TGF-β1 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC000125 |
| Gene Symbol | TGFB1 |
| Gene ID (NCBI) | 7040 |
| RRID | AB_2811115 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01137 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1).
This antibody detects the pro-TGF beta 1 and the cleaved fragment Latency-associated peptide.
This antibody detects the pro-TGF beta 1 and the cleaved fragment Latency-associated peptide.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TGF Beta 1 antibody 21898-1-AP | Download protocol |
| IF protocol for TGF Beta 1 antibody 21898-1-AP | Download protocol |
| IP protocol for TGF Beta 1 antibody 21898-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Mater Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine | ||
ACS Nano Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA. | ||
Blood C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse | ||
Acta Pharm Sin B Fucoidan-functionalized activated platelet-hitchhiking micelles simultaneously track tumor cells and remodel the immunosuppressive microenvironment for efficient metastatic cancer treatment. | ||
J Exp Clin Cancer Res ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK | ||
Autophagy Trehalose induces autophagy via lysosomal-mediated TFEB activation in models of motoneuron degeneration. |
