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TGF Beta 1 Recombinant antibody

TGF Beta 1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230544B7

Cat no : 81746-2-RR

Print datasheet

Synonyms

TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, Tgfb 1, TGFB

验证数据展示

  • WB analysis using 81746-2-RR

    WB analysis using 81746-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81746-2-RR (TGFB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 81746-2-RR

    IHC staining of mouse lung using 81746-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TGF beta 1 antibody included
  • IHC staining of mouse lung using 81746-2-RR

    IHC staining of mouse lung using 81746-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TGF beta 1 antibody included
  • IF Staining of HEK-293 using 81746-2-RR

    IF Staining of HEK-293 using 81746-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TGFB1 antibody (81746-2-RR, Clone: 230544B7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • FC experiment of HEK-293 using 81746-2-RR

    FC experiment of HEK-293 using 81746-2-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug TGF Beta 1 Recombinant antibody (81746-2-RR, Clone:230544B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 81746-2-RR

    Affinity and Kinetic Characterization of 81746-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81746-2-RR against Human TGFB1 were performed. The affinity constant is 9.65 nM.

经过测试的应用

Positive WB detected in4T1 cells, K-562 cells, L-929 cells, RAW 264.7 cells, mouse spleen tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy TGF beta 1 Ready-To-Use IHC Kit. TGF beta 1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 81746-2-RR is TGF Beta 1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen TGF Beta 1 fusion protein Ag13591 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transforming growth factor, beta 1
Synonyms TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, Tgfb 1, TGFB
Calculated Molecular Weight 44 kDa
Observed Molecular Weight44 kDa
GenBank Accession NumberBC000125
Gene Symbol TGFB1
Gene ID (NCBI) 7040
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01137
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1). Full-length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB (12, 25, 45 to 65 kDa).

实验方案

Product Specific Protocols
WB protocol for TGF Beta 1 antibody 81746-2-RRDownload protocol
IHC protocol for TGF Beta 1 antibody 81746-2-RRDownload protocol
IF protocol for TGF Beta 1 antibody 81746-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols