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  • KD/KO Validated

TGFBR2 Monoclonal antibody

TGFBR2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat and More (4)

Applications

WB, IHC, ELISA and More (3)

Conjugate

Unconjugated

CloneNo.

2D5H7

Cat no : 66636-1-Ig

Print datasheet

Synonyms

AAT3, FAA3, LDS1B, LDS2B, MFS2, RIIC, TAAD2, TbetaR II, TGF beta receptor type 2, TGF beta receptor type II, TGF beta type II receptor, TGFbeta RII, TGFBR2, TGFR 2



经过测试的应用

Positive WB detected inA549 cells, HEK-293T cells, U2OS cells, THP-1 cells, HSC-T6 cells, NIH/3T3 cells, NCCIT cells, L02 cells, K-562 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66636-1-Ig targets TGFBR2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity Human, Mouse , Rat
Cited Reactivityhuman, mouse, rat, goat
Immunogen TGFBR2 fusion protein Ag25773 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name transforming growth factor, beta receptor II (70/80kDa)
Synonyms AAT3, FAA3, LDS1B, LDS2B, MFS2, RIIC, TAAD2, TbetaR II, TGF beta receptor type 2, TGF beta receptor type II, TGF beta type II receptor, TGFbeta RII, TGFBR2, TGFR 2
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 65-80 kDa
GenBank Accession NumberBC040499
Gene Symbol TGFBR2
Gene ID (NCBI) 7048
RRIDAB_2881995
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP37173
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TGF beta Receptor II (TGFBR2) is a member of the transforming growth factor-β (TGF-β) superfamily, which are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (PMID: 10974075). TGFBR2 result in both SMAD4-dependent constraint of proliferation and SMAD4-independent activation of apoptosis (PMID: 29396446). It has some isoforms with the molecular weight of 70-80 kDa and ~90 kDa after glycosylated.

实验方案

Product Specific Protocols
WB protocol for TGFBR2 antibody 66636-1-IgDownload protocol
IHC protocol for TGFBR2 antibody 66636-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Rep Med

Engineering irradiated tumor-derived microparticles as personalized vaccines to enhance anti-tumor immunity

Authors - Yajie Sun
mouse,ratIF,WB,IHC

Acta Pharmacol Sin

GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy

Authors - Ji-Chao Wu
ratWB,IP

Br J Pharmacol

Inhibition of HSP90 S-nitrosylation Alleviates Cardiac Fibrosis via TGFβ/SMAD3 Signaling Pathway.

Authors - Xiyue Zhang
ratIHC

NPJ Regen Med

Alkaline shear-thinning micro-nanocomposite hydrogels initiate endogenous TGFβ signaling for in situ bone regeneration

Authors - Yuting Niu
human,mouseWB,IHC

Acta Pharmacol Sin

N-n-Butyl haloperidol iodide ameliorates liver fibrosis and hepatic stellate cell activation in mice.

Authors - Dai-Fei Shen
  • KD Validated
humanWB,IP

Aging (Albany NY)

A novel ALG10/TGF-β positive regulatory loop contributes to the stemness of colorectal cancer.

Authors - Xiaotian Xu