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  • Featured Product
  • KD/KO Validated

TGFBR2 Monoclonal antibody, PBS Only

TGFBR2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D5H7

Cat No : 66636-1-PBS

Print datasheet

Synonyms

AAT3, FAA3, LDS1B, LDS2B, MFS2, RIIC, TAAD2, TbetaR II, TGF beta receptor type 2, TGF beta receptor type II, TGF beta type II receptor, TGFbeta RII, TGFBR2, TGFR 2

验证数据展示

  • WB analysis using 66636-1-Ig (same clone as 66636-1-PBS)

    WB analysis using 66636-1-Ig (same clone as 66636-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293T using 66636-1-Ig (same clone as 66636-1-PBS)

    WB analysis of HEK-293T using 66636-1-Ig (same clone as 66636-1-PBS)

    WB result of TGFBR2 antibody (66636-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGFBR2 transfected HEK-293T cells. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66636-1-Ig (same clone as 66636-1-PBS)

    IHC staining of human breast cancer using 66636-1-Ig (same clone as 66636-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66636-1-Ig (TGFBR2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66636-1-Ig (same clone as 66636-1-PBS)

    IHC staining of human breast cancer using 66636-1-Ig (same clone as 66636-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66636-1-Ig (TGFBR2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

产品信息

66636-1-PBS targets TGFBR2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse , Rat
Immunogen TGFBR2 fusion protein Ag25773 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name transforming growth factor, beta receptor II (70/80kDa)
Synonyms AAT3, FAA3, LDS1B, LDS2B, MFS2, RIIC, TAAD2, TbetaR II, TGF beta receptor type 2, TGF beta receptor type II, TGF beta type II receptor, TGFbeta RII, TGFBR2, TGFR 2
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 65-80 kDa
GenBank Accession NumberBC040499
Gene Symbol TGFBR2
Gene ID (NCBI) 7048
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP37173
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

TGF beta Receptor II (TGFBR2) is a member of the transforming growth factor-β (TGF-β) superfamily, which are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (PMID: 10974075). TGFBR2 result in both SMAD4-dependent constraint of proliferation and SMAD4-independent activation of apoptosis (PMID: 29396446). It has some isoforms with the molecular weight of 70-80 kDa and ~90 kDa after glycosylated.