验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human placenta tissue, HUVEC cells, HepG2 cells, K-562 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy TGM2 Ready-To-Use IHC Kit. TGM2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68006-1-Ig targets TGM2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | TGM2 fusion protein Ag7462 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase) |
| Synonyms | TGase C, TGase 2, TG2, TG(C), Erythrocyte transglutaminase |
| Calculated Molecular Weight | 77 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC003551 |
| Gene Symbol | TGM2 |
| Gene ID (NCBI) | 7052 |
| ENSEMBL Gene ID | ENSG00000198959 |
| RRID | AB_2918753 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P21980 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TGM2 antibody 68006-1-Ig | Download protocol |
| IHC protocol for TGM2 antibody 68006-1-Ig | Download protocol |
| IF protocol for TGM2 antibody 68006-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomaterials Tumor-specific nitric oxide generator to amplify peroxynitrite based on highly penetrable nanoparticles for metastasis inhibition and enhanced cancer therapy. | ||
Transl Res Counteracting TGM2 by a Fibroin peptide ameliorated Adriamycin-induced nephropathy via regulation of lipid metabolism through PANX1-PPAR α/PANK1 pathway | ||
F1000Res A guide to selecting high-performing antibodies for Protein-glutamine gamma-glutamyltransferase 2 (TGM2) for use in western blot, immunoprecipitation and immunofluorescence |
