验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, human placenta tissue, HUVEC cells, HepG2 cells, K-562 cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68006-1-Ig targets TGM2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human |
文献引用反应性 | human, mouse |
免疫原 | TGM2 fusion protein Ag7462 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase) |
别名 | TGase C, TGase 2, TG2, TG(C), Erythrocyte transglutaminase |
计算分子量 | 77 kDa |
观测分子量 | 80 kDa |
GenBank蛋白编号 | BC003551 |
基因名称 | TGM2 |
Gene ID (NCBI) | 7052 |
ENSEMBL Gene ID | ENSG00000198959 |
RRID | AB_2918753 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P21980 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for TGM2 antibody 68006-1-Ig | Download protocol |
IHC protocol for TGM2 antibody 68006-1-Ig | Download protocol |
IF protocol for TGM2 antibody 68006-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Biomaterials Tumor-specific nitric oxide generator to amplify peroxynitrite based on highly penetrable nanoparticles for metastasis inhibition and enhanced cancer therapy. | ||
Transl Res Counteracting TGM2 by a Fibroin peptide ameliorated Adriamycin-induced nephropathy via regulation of lipid metabolism through PANX1-PPAR α/PANK1 pathway | ||
F1000Res A guide to selecting high-performing antibodies for Protein-glutamine gamma-glutamyltransferase 2 (TGM2) for use in western blot, immunoprecipitation and immunofluorescence |