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TGM2 Monoclonal antibody, PBS Only

TGM2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D4C11

Cat no : 68006-1-PBS

Print datasheet

Synonyms

TGase C, TGase 2, TG2, TG(C), Erythrocyte transglutaminase

验证数据展示

  • WB analysis of HeLa using 68006-1-Ig (same clone as 68006-1-PBS)

    WB analysis of HeLa using 68006-1-Ig (same clone as 68006-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68006-1-Ig (TGM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 68006-1-Ig (same clone as 68006-1-PBS)

    IHC staining of human liver cancer using 68006-1-Ig (same clone as 68006-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68006-1-Ig (TGM2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TGM2 antibody included
  • IF Staining of A549 using 68006-1-Ig (same clone as 68006-1-PBS)

    IF Staining of A549 using 68006-1-Ig (same clone as 68006-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TGM2 antibody (68006-1-Ig, Clone: 2D4C11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68006-1-Ig (same clone as 68006-1-PBS)

    FC experiment of HeLa using 68006-1-Ig (same clone as 68006-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TGM2 (68006-1-Ig, Clone:2D4C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TGM2 Ready-To-Use IHC Kit. TGM2 primary antibody included.

产品信息

68006-1-PBS targets TGM2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen TGM2 fusion protein Ag7462 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
Synonyms TGase C, TGase 2, TG2, TG(C), Erythrocyte transglutaminase
Calculated Molecular Weight 77 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC003551
Gene Symbol TGM2
Gene ID (NCBI) 7052
ENSEMBL Gene IDENSG00000198959
RRIDAB_2918753
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21980
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C