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TGN46 Monoclonal antibody, PBS Only

TGN46 Monoclonal Antibody for WB,Indirect ELISA,IHC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig, Mouse

Applications

WB,Indirect ELISA,IHC

Conjugate

Unconjugated

CloneNo.

1F6D5

Cat No : 66477-1-PBS

Print datasheet

Synonyms

Golgi marker, TGN38, TGN38 homolog, TGN46, TGN48, TGN51, TGOLN2, trans golgi network protein 2, TTGN2

验证数据展示

  • WB analysis of HeLa using 66477-1-Ig (same clone as 66477-1-PBS)

    WB analysis of HeLa using 66477-1-Ig (same clone as 66477-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

  • WB analysis using 66477-1-Ig (same clone as 66477-1-PBS)

    WB analysis using 66477-1-Ig (same clone as 66477-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66477-1-Ig (same clone as 66477-1-PBS)

    IHC staining of human breast cancer using 66477-1-Ig (same clone as 66477-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66477-1-Ig (same clone as 66477-1-PBS)

    IHC staining of human breast cancer using 66477-1-Ig (same clone as 66477-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

产品信息

66477-1-PBS targets TGN46 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Pig, Mouse samples.

Tested Applications WB,Indirect ELISA,IHC Application Description
Tested Reactivity Human, Rat, Pig, Mouse
Immunogen TGN46 fusion protein Ag4470 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name trans-golgi network protein 2
Synonyms Golgi marker, TGN38, TGN38 homolog, TGN46, TGN48, TGN51, TGOLN2, trans golgi network protein 2, TTGN2
Calculated Molecular Weight 447 aa, 47 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC028219
Gene Symbol TGOLN2
Gene ID (NCBI) 10618
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43493
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.