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验证数据展示

  • WB analysis of HeLa using 66477-1-Ig (same clone as 66477-1-PBS)

    WB analysis of HeLa using 66477-1-Ig (same clone as 66477-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

  • WB analysis using 66477-1-Ig (same clone as 66477-1-PBS)

    WB analysis using 66477-1-Ig (same clone as 66477-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66477-1-Ig (same clone as 66477-1-PBS)

    IHC staining of human breast cancer using 66477-1-Ig (same clone as 66477-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66477-1-Ig (same clone as 66477-1-PBS)

    IHC staining of human breast cancer using 66477-1-Ig (same clone as 66477-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

TGN46 Monoclonal antibody, PBS Only

TGN46 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66477-1-PBS

产品说明书

CloneNo. 1F6D5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, Indirect ELISA

别名

TGOLN2, TGN38 homolog, TGN38, hTGN51, hTGN48

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66477-1-PBS targets TGN46 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 TGN46 fusion protein Ag4470 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 trans-golgi network protein 2
别名 TGOLN2, TGN38 homolog, TGN38, hTGN51, hTGN48
计算分子量 447 aa, 47 kDa
观测分子量 90 kDa
GenBank蛋白编号BC028219
基因名称 TGN46
Gene ID (NCBI) 10618
RRIDAB_2881843
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43493
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.