验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, LNCaP cells, HepG2 cells, L02 cells, SMMC-7721 cells, human colostrum tissue, pig liver tissue |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66477-1-Ig targets TGN46 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 | TGN46 fusion protein Ag4470 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | trans-golgi network protein 2 |
| 别名 | TGOLN2, TGN38 homolog, TGN38, hTGN51, hTGN48 |
| 计算分子量 | 447 aa, 47 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC028219 |
| 基因名称 | TGN46 |
| Gene ID (NCBI) | 10618 |
| RRID | AB_2881843 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O43493 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TGN46 antibody 66477-1-Ig | Download protocol |
| IHC protocol for TGN46 antibody 66477-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Arterioscler Thromb Vasc Biol IL-1β Induces LDL Transcytosis by a Novel Pathway Involving LDLR and Rab27a | ||
J Virol Porcine deltacoronavirus accessory protein NS6 harnesses VPS35-mediated retrograde trafficking to facilitate efficient viral infection | ||
Int J Biol Sci Annexin A5 Derived from Cell-free Fat Extract Attenuates Osteoarthritis via Macrophage Regulation |
