• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83099-2-RR (same clone as 83099-2-PBS)

    WB analysis using 83099-2-RR (same clone as 83099-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83099-2-RR (TGN46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83099-2-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 83099-2-RR (same clone as 83099-2-PBS)

    IHC staining of human stomach cancer using 83099-2-RR (same clone as 83099-2-PBS)

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 83099-2-RR (TGN46 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83099-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83099-2-RR (same clone as 83099-2-PBS)

    IF Staining of HeLa using 83099-2-RR (same clone as 83099-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TGN46 antibody (83099-2-RR, Clone: 230376D6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83099-2-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83099-2-RR (same clone as 83099-2-PBS)

    FC experiment of HepG2 using 83099-2-RR (same clone as 83099-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug TGOLN2,TGN46 Recombinant antibody (83099-2-RR, Clone:230376D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83099-2-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 83099-2-RR (same clone as 83099-2-PBS)

    IP experiment of HeLa using 83099-2-RR (same clone as 83099-2-PBS)

    IP result of anti-TGN46 (IP:83099-2-RR, 4ug; Detection:83099-2-RR 1:2000) with HeLa cells lysate 980 ug. This data was developed using the same antibody clone with 83099-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83099-2-RR (same clone as 83099-2-PBS)

    IF Staining of HeLa using 83099-2-RR (same clone as 83099-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TGOLN2,TGN46 antibody (83099-2-RR, Clone: 230376D6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83099-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83099-2-RR

    Affinity and Kinetic Characterization of 83099-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83099-2-RR against Human TGOLN2,TGN46 were performed. The affinity constant is 0.633 nM.

TGN46 Recombinant antibody, PBS Only

TGN46 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 83099-2-PBS

产品说明书

CloneNo. 230376D6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

TGOLN2,TGN46, TGOLN2, TGN38 homolog, TGN38, hTGN51

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

83099-2-PBS targets TGN46 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 TGN46 fusion protein Ag4470 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 trans-golgi network protein 2
别名 TGOLN2,TGN46, TGOLN2, TGN38 homolog, TGN38, hTGN51
计算分子量 447 aa, 47 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC028219
基因名称 TGN46
Gene ID (NCBI) 10618
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43493
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.