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验证数据展示

  • WB analysis using 83099-2-RR

    WB analysis using 83099-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83099-2-RR (TGN46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 83099-2-RR

    IHC staining of human stomach cancer using 83099-2-RR

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 83099-2-RR (TGN46 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 83099-2-RR

    IF Staining of HeLa using 83099-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TGN46 antibody (83099-2-RR, Clone: 230376D6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HepG2 using 83099-2-RR

    FC experiment of HepG2 using 83099-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug TGOLN2,TGN46 Recombinant antibody (83099-2-RR, Clone:230376D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IP experiment of HeLa using 83099-2-RR

    IP experiment of HeLa using 83099-2-RR

    IP result of anti-TGN46 (IP:83099-2-RR, 4ug; Detection:83099-2-RR 1:2000) with HeLa cells lysate 980 ug.

  • IF Staining of HeLa using 83099-2-RR

    IF Staining of HeLa using 83099-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TGOLN2,TGN46 antibody (83099-2-RR, Clone: 230376D6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83099-2-RR

    Affinity and Kinetic Characterization of 83099-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83099-2-RR against Human TGOLN2,TGN46 were performed. The affinity constant is 0.633 nM.

TGN46 Recombinant antibody

TGN46 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 83099-2-RR

产品说明书

CloneNo. 230376D6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

TGOLN2,TGN46, TGOLN2, TGN38 homolog, TGN38, hTGN51

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, PC-3 cells, PC-12 cells, human kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83099-2-RR targets TGN46 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原 TGN46 fusion protein Ag4470 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 trans-golgi network protein 2
别名 TGOLN2,TGN46, TGOLN2, TGN38 homolog, TGN38, hTGN51
计算分子量 447 aa, 47 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC028219
基因名称 TGN46
Gene ID (NCBI) 10618
RRIDAB_3670812
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43493
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

实验方案

Product Specific Protocols
WB protocol for TGN46 antibody 83099-2-RRDownload protocol
IHC protocol for TGN46 antibody 83099-2-RRDownload protocol
IF protocol for TGN46 antibody 83099-2-RRDownload protocol
IP protocol for TGN46 antibody 83099-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols