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验证数据展示

  • IF Staining of HeLa using CL488-83099-2

    IF Staining of HeLa using CL488-83099-2

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TGN46 antibody (CL488-83099-2, Clone: 230376D6 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-83099-2

    FC experiment of HepG2 using CL488-83099-2

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated TGN46 Recombinant antibody (CL488-83099-2, Clone:230376D6)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated TGN46 Recombinant antibody

TGN46 Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-83099-2
CloneNo. 230376D6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

TGOLN2, TGN38 homolog, TGN38, hTGN51, hTGN48

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 83099-2-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83099-2 targets TGN46 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen TGN46 fusion protein Ag4470 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name trans-golgi network protein 2
Synonyms TGOLN2, TGN38 homolog, TGN38, hTGN51, hTGN48
Calculated Molecular Weight 447 aa, 47 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC028219
Gene Symbol TGN46
Gene ID (NCBI) 10618
RRIDAB_3673189
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43493
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TGN46 antibody CL488-83099-2Download protocol
Standard Protocols
Click here to view our Standard Protocols