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验证数据展示

  • IF Staining of HeLa using CL488-83099-2

    IF Staining of HeLa using CL488-83099-2

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TGN46 antibody (CL488-83099-2, Clone: 230376D6 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-83099-2

    FC experiment of HepG2 using CL488-83099-2

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated TGN46 Recombinant antibody (CL488-83099-2, Clone:230376D6)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated TGN46 Recombinant antibody

TGN46 Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-83099-2

产品说明书

CloneNo. 230376D6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

TGOLN2, TGN38 homolog, TGN38, hTGN51, hTGN48

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 83099-2-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83099-2 targets TGN46 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 TGN46 fusion protein Ag4470 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 trans-golgi network protein 2
别名 TGOLN2, TGN38 homolog, TGN38, hTGN51, hTGN48
计算分子量 447 aa, 47 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC028219
基因名称 TGN46
Gene ID (NCBI) 10618
RRIDAB_3673189
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43493
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TGN46 antibody CL488-83099-2Download protocol
Standard Protocols
Click here to view our Standard Protocols