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验证数据展示

  • IF Staining of HeLa using CL594-67380

    IF Staining of HeLa using CL594-67380

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 TH1L antibody (CL594-67380, Clone: 1A10G2 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-67380

    FC experiment of HeLa using CL594-67380

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human TH1L (CL594-67380, Clone:1A10G2) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated TH1L Monoclonal antibody

TH1L Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67380

Print datasheet

CloneNo. 1A10G2

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

HSPC130, Negative elongation factor C/D, NELF C, NELF C/D, NELF D, NELFD, TH1, TH1 like (Drosophila), TH1 like protein, TH1L

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NELFCD/TH1L Ready-To-Use IHC Kit. NELFCD/TH1L primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67380-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67380 targets TH1L in IF/ICC, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity Human, mouse, rat
Immunogen TH1L fusion protein Ag24894 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name TH1-like (Drosophila)
Synonyms HSPC130, Negative elongation factor C/D, NELF C, NELF C/D, NELF D, NELFD, TH1, TH1 like (Drosophila), TH1 like protein, TH1L
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 60-66 kDa
GenBank Accession NumberBC014952
Gene Symbol TH1L
Gene ID (NCBI) 51497
RRIDAB_3084762
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IXH7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 TH1L antibody CL594-67380Download protocol
FC protocol for CL594 TH1L antibody CL594-67380Download protocol
Standard Protocols
Click here to view our Standard Protocols