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CTMP Polyclonal antibody

CTMP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14692-1-AP

Print datasheet

Synonyms

THEM4, EC:3.1.2.2, Carboxyl-terminal modulator protein, Acyl-coenzyme A thioesterase THEM4, Acyl-CoA thioesterase THEM4

验证数据展示

  • WB analysis of mouse brain using 14692-1-AP

    WB analysis of mouse brain using 14692-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14692-1-AP (CTMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human endometrial cancer using 14692-1-AP

    IHC staining of human endometrial cancer using 14692-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14692-1-AP (CTMP antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human endometrial cancer using 14692-1-AP

    IHC staining of human endometrial cancer using 14692-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14692-1-AP (CTMP antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HeLa using 14692-1-AP

    IF Staining of HeLa using 14692-1-AP

    Immunofluorescent analysis of Hela cells, using CTMP antibody 14692-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of mouse brain using 14692-1-AP

    IP experiment of mouse brain using 14692-1-AP

    IP result of anti-CTMP (IP:14692-1-AP, 4ug; Detection:14692-1-AP 1:500) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse brain tissue, HepG2 cells, mouse skeletal muscle tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14692-1-AP targets CTMP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen CTMP fusion protein Ag6244 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thioesterase superfamily member 4
Synonyms THEM4, EC:3.1.2.2, Carboxyl-terminal modulator protein, Acyl-coenzyme A thioesterase THEM4, Acyl-CoA thioesterase THEM4
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC065277
Gene Symbol CTMP
Gene ID (NCBI) 117145
RRIDAB_2271685
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T1C6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CTMP antibody 14692-1-APDownload protocol
IHC protocol for CTMP antibody 14692-1-APDownload protocol
IF protocol for CTMP antibody 14692-1-APDownload protocol
IP protocol for CTMP antibody 14692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Heliyon

PRAME promotes proliferation of multiple myeloma cells through CTMP/Akt/p21/CCND3 axis by ubiquitinating CTMP and p21

Authors - Kai Sun
View Article