• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82672-1-RR (same clone as 82672-1-PBS)

    WB analysis using 82672-1-RR (same clone as 82672-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82672-1-RR (TIA1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82672-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 82672-1-RR (same clone as 82672-1-PBS)

    WB analysis of Jurkat using 82672-1-RR (same clone as 82672-1-PBS)

    WB result of TIA1 antibody (82672-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TIA1 transfected Jurkat cells. This data was developed using the same antibody clone with 82672-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82672-1-RR (same clone as 82672-1-PBS)

    IF Staining of HeLa using 82672-1-RR (same clone as 82672-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using TIA1 antibody (82672-1-RR, Clone: 3N22 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 82672-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82672-1-RR (same clone as 82672-1-PBS)

    IF Staining of HeLa using 82672-1-RR (same clone as 82672-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using TIA1 antibody (82672-1-RR, Clone: 3N22 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82672-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82672-1-PBS

    Affinity and Kinetic Characterization of 82672-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82672-1-RR against Human TIA1 were performed. The affinity constant is 0.385 nM.

TIA1 Recombinant antibody, PBS Only

TIA1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 82672-1-PBS

产品说明书

CloneNo. 3N22

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

RNA binding protein TIA 1, p40-TIA-1, p40 TIA 1, Nucleolysin TIA-1 isoform p40, Cytotoxic granule associated RNA binding protein TIA1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy TIA1 Ready-To-Use IHC Kit. TIA1 primary antibody included.

产品信息

82672-1-PBS targets TIA1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 TIA1 fusion protein Ag2778 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TIA1 cytotoxic granule-associated RNA binding protein
别名 RNA binding protein TIA 1, p40-TIA-1, p40 TIA 1, Nucleolysin TIA-1 isoform p40, Cytotoxic granule associated RNA binding protein TIA1
计算分子量 214 aa, 24 kDa, 43 kDa
观测分子量38-40 kDa
GenBank蛋白编号BC015944
基因名称 TIA1
Gene ID (NCBI) 7072
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP31483
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TIA1, also named as p40-TIA-1, is involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). It possesses nucleolytic activity against cytotoxic lymphocyte target cells. TIA1 may be involved in apoptosis. Two isoforms of this protein exist - 41kDa and 42kDa. one of these was a missense variant (P362L) in TIA1. Similar to the ALS-related disease proteins TDP-43, hnRNPA1, and FUS, TIA1 is an RNA-binding protein containing a prionlike LCD and assembles into membrane-less organelles, including SGs. Postmortem neuropathology of five TIA1 mutations carriers showed a consistent pathological signature with numerous round, hyaline, TAR DNA-binding protein 43 (TDP-43)-positive inclusions.TIA1mutations significantly increased the propensity of TIA1 protein to undergo phase transition. In live cells,TIA1mutations delayed stress granule (SG) disassembly and promoted the accumulation of non-dynamic SGs that harbored TDP-43.