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TIA1 Recombinant antibody
TIA1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
3N22
验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells, Jurkat cells, COLO 320 cells, K-562 cells, MOLT-4 cells, NIH/3T3 cells, HSC-T6 cells |
| Positive IF/ICC detected in | sodium arsenite treated HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy TIA1 Ready-To-Use IHC Kit. TIA1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82672-1-RR targets TIA1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Tested Reactivity | Human, mouse, rat |
| Immunogen | TIA1 fusion protein Ag2778 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | TIA1 cytotoxic granule-associated RNA binding protein |
| Synonyms | RNA binding protein TIA 1, p40-TIA-1, p40 TIA 1, Nucleolysin TIA-1 isoform p40, Cytotoxic granule associated RNA binding protein TIA1 |
| Calculated Molecular Weight | 214 aa, 24 kDa, 43 kDa |
| Observed Molecular Weight | 38-40 kDa |
| GenBank Accession Number | BC015944 |
| Gene Symbol | TIA1 |
| Gene ID (NCBI) | 7072 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P31483 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TIA1, also named as p40-TIA-1, is involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). It possesses nucleolytic activity against cytotoxic lymphocyte target cells. TIA1 may be involved in apoptosis. Two isoforms of this protein exist - 41kDa and 42kDa. one of these was a missense variant (P362L) in TIA1. Similar to the ALS-related disease proteins TDP-43, hnRNPA1, and FUS, TIA1 is an RNA-binding protein containing a prionlike LCD and assembles into membrane-less organelles, including SGs. Postmortem neuropathology of five TIA1 mutations carriers showed a consistent pathological signature with numerous round, hyaline, TAR DNA-binding protein 43 (TDP-43)-positive inclusions.TIA1mutations significantly increased the propensity of TIA1 protein to undergo phase transition. In live cells,TIA1mutations delayed stress granule (SG) disassembly and promoted the accumulation of non-dynamic SGs that harbored TDP-43.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TIA1 antibody 82672-1-RR | Download protocol |
| IF protocol for TIA1 antibody 82672-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |