验证数据展示
经过测试的应用
Positive WB detected in | PC-3 cells, THP-1 cells, Daudi cells, RAW 264.7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:7000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82836-1-RR targets TIM3 in WB, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | TIM3 fusion protein Ag2459 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | hepatitis A virus cellular receptor 2 |
别名 | T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366 |
计算分子量 | 301 aa, 33 kDa |
观测分子量 | 70 kDa |
GenBank蛋白编号 | BC020843 |
基因名称 | TIM3 |
Gene ID (NCBI) | 84868 |
RRID | AB_3670571 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q8TDQ0 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).
实验方案
Product Specific Protocols | |
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WB protocol for TIM3 antibody 82836-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |