TIM3 Recombinant antibody
TIM3 Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
5A8
验证数据展示
经过测试的应用
Positive WB detected in | PC-3 cells, THP-1 cells, Daudi cells, RAW 264.7 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:7000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82836-1-RR targets TIM3 in WB, ELISA applications and shows reactivity with Human, Mouse samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | Human, Mouse |
Immunogen | TIM3 fusion protein Ag2459 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | hepatitis A virus cellular receptor 2 |
Synonyms | T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366 |
Calculated Molecular Weight | 301 aa, 33 kDa |
Observed Molecular Weight | 70 kDa |
GenBank Accession Number | BC020843 |
Gene Symbol | TIM3 |
Gene ID (NCBI) | 84868 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q8TDQ0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).
实验方案
Product Specific Protocols | |
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WB protocol for TIM3 antibody 82836-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |