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TIM3 Recombinant antibody, PBS Only

TIM3 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5A8

Cat no : 82836-1-PBS

Print datasheet

Synonyms

T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366

验证数据展示

  • WB analysis using 82836-1-RR (same clone as 82836-1-PBS)

    WB analysis using 82836-1-RR (same clone as 82836-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82836-1-RR (TIM3 antibody) at dilution of 1:3500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82836-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82836-1-PBS

    Affinity and Kinetic Characterization of 82836-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82836-1-RR against Human TIM3 were performed. The affinity constant is below 1 pM.

产品信息

82836-1-PBS targets TIM3 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen TIM3 fusion protein Ag2459 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name hepatitis A virus cellular receptor 2
Synonyms T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366
Calculated Molecular Weight 301 aa, 33 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC020843
Gene Symbol TIM3
Gene ID (NCBI) 84868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TDQ0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).