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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 82836-1-RR (same clone as 82836-1-PBS)

    WB analysis using 82836-1-RR (same clone as 82836-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82836-1-RR (TIM3 antibody) at dilution of 1:3500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82836-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 82836-1-RR (same clone as 82836-1-PBS)

    WB analysis of PC-3 using 82836-1-RR (same clone as 82836-1-PBS)

    WB result of TIM3 antibody (82836-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TIM3 transfected PC-3 cells. This data was developed using the same antibody clone with 82836-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82836-1-RR

    Affinity and Kinetic Characterization of 82836-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82836-1-RR against Human TIM3 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

TIM3 Recombinant antibody, PBS Only

TIM3 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 82836-1-PBS

产品说明书

CloneNo. 5A8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82836-1-PBS targets TIM3 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 TIM3 fusion protein Ag2459 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hepatitis A virus cellular receptor 2
别名 T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366
计算分子量 301 aa, 33 kDa
观测分子量70 kDa
GenBank蛋白编号BC020843
基因名称 TIM3
Gene ID (NCBI) 84868
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8TDQ0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).