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Anti-Human TIM3 Rabbit Recombinant Antibody, PBS Only

TIM3 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241422H1

Cat no : 98115-1-PBS

Print datasheet

Synonyms

T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366

验证数据展示

  • FC experiment of human PBMCs using 98115-1-RR (same clone as 98115-1-PBS)

    FC experiment of human PBMCs using 98115-1-RR (same clone as 98115-1-PBS)

    1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were surface stained with 0.25 ug Anti-Human TIM3 Rabbit Recombinant Antibody (98115-1-RR, Clone: 241422H1) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD4 (RPA-T4) (APC-65143, Clone: RPA-T4). Cells were not fixed. This data was developed using the same antibody clone with 98115-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98115-1-RR (same clone as 98115-1-PBS)

    FC experiment of human PBMCs using 98115-1-RR (same clone as 98115-1-PBS)

    1x10^6 anti-CD3/CD28 treated human PBMCs were surface stained with 0.25 ug Anti-Human TIM3 Rabbit Recombinant Antibody (98115-1-RR, Clone: 241422H1) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human TIM3 Rabbit Recombinant Antibody (98115-1-RR, Clone: 241422H1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed. This data was developed using the same antibody clone with 98115-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98115-1-RR

    Affinity and Kinetic Characterization of 98115-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98115-1-RR against Human TIM3 were performed. The affinity constant is 0.688 nM.

产品信息

98115-1-PBS targets TIM3 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name hepatitis A virus cellular receptor 2
Synonyms T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366
Calculated Molecular Weight 301 aa, 33 kDa
GenBank Accession NumberBC020843
Gene Symbol TIM3
Gene ID (NCBI) 84868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8TDQ0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920).