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验证数据展示

  • FC experiment of human PBMCs using 98115-1-RR

    FC experiment of human PBMCs using 98115-1-RR

    1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were surface stained with 0.25 ug Anti-Human TIM3 Rabbit Recombinant Antibody (98115-1-RR, Clone: 241422H1) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD4 (RPA-T4) (APC-65143, Clone: RPA-T4). Cells were not fixed.

  • FC experiment of human PBMCs using 98115-1-RR

    FC experiment of human PBMCs using 98115-1-RR

    1x10^6 anti-CD3/CD28 treated human PBMCs were surface stained with 0.25 ug Anti-Human TIM3 Rabbit Recombinant Antibody (98115-1-RR, Clone: 241422H1) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human TIM3 Rabbit Recombinant Antibody (98115-1-RR, Clone: 241422H1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98115-1-RR

    Affinity and Kinetic Characterization of 98115-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98115-1-RR against Human TIM3 were performed. The affinity constant is 0.688 nM.

Anti-Human TIM3 Rabbit Recombinant Antibody

TIM3 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98115-1-RR

产品说明书

CloneNo. 241422H1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inAnti-CD3/CD28 treated human PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98115-1-RR targets TIM3 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hepatitis A virus cellular receptor 2
别名 T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366
计算分子量 301 aa, 33 kDa
GenBank蛋白编号BC020843
基因名称 TIM3
Gene ID (NCBI) 84868
RRIDAB_3672262
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8TDQ0
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920).

实验方案

Product Specific Protocols
FC protocol for TIM3 antibody 98115-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols