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验证数据展示

  • IF Staining of human tonsillitis using CL488-60355

    IF Staining of human tonsillitis using CL488-60355

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CoraLite® Plus 488 TIM3 antibody (CL488-60355, Clone: 4C4G3 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 488-conjugated TIM3 Monoclonal antibody

TIM3 Monoclonal Antibody for IF-P
Cat No. CL488-60355

产品说明书

CloneNo. 4C4G3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF-P

别名

T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
For other applications, we recommend the unconjugated version of this antibody, 60355-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60355 targets TIM3 in IF-P applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat, pig
免疫原 TIM3 fusion protein Ag16901 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 hepatitis A virus cellular receptor 2
别名 T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366
计算分子量 301 aa, 33 kDa
观测分子量 33 kDa, 50-70 kDa
GenBank蛋白编号BC020843
基因名称 TIM3
Gene ID (NCBI) 84868
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8TDQ0
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa detected by this monoclonal antibody probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TIM3 antibody CL488-60355Download protocol
Standard Protocols
Click here to view our Standard Protocols