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  • Featured Product
  • KD/KO Validated

TIMELESS Monoclonal antibody, PBS Only

TIMELESS Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B6G3

Cat no : 67022-1-PBS

Print datasheet

Synonyms

TIMELESS1, timeless homolog (Drosophila), TIM1, TIM, Protein timeless homolog

验证数据展示

  • WB analysis using 67022-1-Ig (same clone as 67022-1-PBS)

    WB analysis using 67022-1-Ig (same clone as 67022-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 67022-1-Ig (same clone as 67022-1-PBS)

    FC experiment of HEK-293 using 67022-1-Ig (same clone as 67022-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human TIMELESS (67022-1-Ig, Clone:1B6G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

产品信息

67022-1-PBS targets TIMELESS in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TIMELESS fusion protein Ag6283 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name timeless homolog (Drosophila)
Synonyms TIMELESS1, timeless homolog (Drosophila), TIM1, TIM, Protein timeless homolog
Calculated Molecular Weight 139 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC050557
Gene Symbol TIMELESS
Gene ID (NCBI) 8914
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UNS1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules.