验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, U2OS cells, A549 cells, JAR cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, K-562 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
67022-1-Ig targets TIMELESS in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, canine |
| 免疫原 | TIMELESS fusion protein Ag6283 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | timeless homolog (Drosophila) |
| 别名 | TIMELESS1, timeless homolog (Drosophila), TIM1, TIM, Protein timeless homolog |
| 计算分子量 | 139 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | BC050557 |
| 基因名称 | TIMELESS |
| Gene ID (NCBI) | 8914 |
| RRID | AB_2882337 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UNS1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TIMELESS antibody 67022-1-Ig | Download protocol |
| FC protocol for TIMELESS antibody 67022-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
