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验证数据展示

  • FC experiment of HEK-293 using CL488-67022

    FC experiment of HEK-293 using CL488-67022

    1X10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human TIMELESS (CL488-67022, Clone:1B6G3) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated TIMELESS Monoclonal antibody

TIMELESS Monoclonal Antibody for FC (Intra)
Cat No. CL488-67022

产品说明书

CloneNo. 1B6G3

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

FC (Intra)

别名

FLJ12640, FLJ20714, hTIM, Protein timeless homolog, TIM, TIM1, TIMELESS, timeless homolog (Drosophila), TIMELESS1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHEK-293 cells
Positive FC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 67022-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67022 targets TIMELESS in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Mouse, Rat
免疫原 TIMELESS fusion protein Ag6283 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 timeless homolog (Drosophila)
别名 FLJ12640, FLJ20714, hTIM, Protein timeless homolog, TIM, TIM1, TIMELESS, timeless homolog (Drosophila), TIMELESS1
计算分子量 139 kDa
观测分子量 140 kDa
GenBank蛋白编号BC050557
基因名称 TIMELESS
Gene ID (NCBI) 8914
RRIDAB_3084299
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UNS1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TIMELESS antibody CL488-67022Download protocol
Standard Protocols
Click here to view our Standard Protocols