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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TIMELESS Monoclonal antibody

TIMELESS Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B6G3

Cat no : CL488-67022

Print datasheet

Synonyms

FLJ12640, FLJ20714, hTIM, Protein timeless homolog, TIM, TIM1, TIMELESS, timeless homolog (Drosophila), TIMELESS1

验证数据展示

  • FC experiment of HEK-293 using CL488-67022

    FC experiment of HEK-293 using CL488-67022

    1X10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human TIMELESS (CL488-67022, Clone:1B6G3) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inHEK-293 cells
Positive FC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 67022-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67022 targets TIMELESS in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen TIMELESS fusion protein Ag6283 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name timeless homolog (Drosophila)
Synonyms FLJ12640, FLJ20714, hTIM, Protein timeless homolog, TIM, TIM1, TIMELESS, timeless homolog (Drosophila), TIMELESS1
Calculated Molecular Weight 139 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC050557
Gene Symbol TIMELESS
Gene ID (NCBI) 8914
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UNS1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TIMELESS antibody CL488-67022Download protocol
Standard Protocols
Click here to view our Standard Protocols