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验证数据展示

  • IF Staining of HepG2 using CL488-66149

    IF Staining of HepG2 using CL488-66149

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 TIMM44 antibody (CL488-66149, Clone: 4G7D4 ) at dilution of 1:200, CL594-Phalloidin (red).

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CoraLite® Plus 488-conjugated TIMM44 Monoclonal antibody

TIMM44 Monoclonal Antibody for IF/ICC
Cat No. CL488-66149

产品说明书

CloneNo. 4G7D4

宿主/亚型

Mouse / IgG2c

种属反应性

human, mouse, rat, pig, rabbit

应用

IF/ICC

别名

TIMM 44, TIM44, MIMT44, 4G7D4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66149-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66149 targets TIMM44 in IF/ICC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 TIMM44 fusion protein Ag21389 种属同源性预测
宿主/亚型 Mouse / IgG2c
抗体类别 Monoclonal
产品类型 Antibody
全称 translocase of inner mitochondrial membrane 44 homolog (yeast)
别名 TIMM 44, TIM44, MIMT44, 4G7D4
计算分子量 452 aa, 51 kDa
观测分子量 45 kDa
GenBank蛋白编号BC033628
基因名称 TIMM44
Gene ID (NCBI) 10469
RRIDAB_3672905
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43615
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Translocase of inner mitochondrial membrane 44 (TIMM44), also known as MIMT44 and TIM44, belongs to the Tim44 family. TIMM44 is a mitochondrial protein located at the inner mitochondrial membrane, which is crucial for the integrity and function of mitochondria. In an ATP-dependent manner, TIMM44 anchors mitochondrial heat shock protein 70 to the translocase of the mitochondrial inner membrane 23 (TIMM23) complex. TIMM44 is essential for mitochondrial pre-protein import into the mitochondrial matrix. TIMM44 is vital for the integrity and function of mitochondria. TIMM44 silencing disrupted mitochondrial functions in endothelial cells, causing mitochondrial protein input arrest, ATP reduction, ROS production, and mitochondrial depolarization, and leading to apoptosis activation (PMID: 36438483, PMID: 37147302, PMID: 38467612). The observed molecular weight of TIMM44 is 45 kDa, indicating a mature form of TIMM44 (PMID: 33891006).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TIMM44 antibody CL488-66149Download protocol
Standard Protocols
Click here to view our Standard Protocols