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TINP1 Monoclonal antibody

TINP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E3B11

Cat no : 66374-1-Ig

Print datasheet

Synonyms

CDK105, Hairy cell leukemia protein 1, HCL G1, HUSSY29, NSA2, TINP1, YR 29

验证数据展示

  • WB analysis of L02 using 66374-1-Ig

    WB analysis of L02 using 66374-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 66374-1-Ig (TINP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 66374-1-Ig

    IHC staining of human breast cancer using 66374-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66374-1-Ig (TINP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 66374-1-Ig

    IHC staining of human breast cancer using 66374-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66374-1-Ig (TINP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 66374-1-Ig

    IF Staining of HEK-293 using 66374-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TINP1 antibody (66374-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of HEK-293 using 66374-1-Ig

    IF Staining of HEK-293 using 66374-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TINP1 antibody (66374-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inL02 cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66374-1-Ig targets TINP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen TINP1 fusion protein Ag8822 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TGF beta-inducible nuclear protein 1
Synonyms CDK105, Hairy cell leukemia protein 1, HCL G1, HUSSY29, NSA2, TINP1, YR 29
Calculated Molecular Weight 260 aa, 30 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC005288
Gene Symbol TINP1
Gene ID (NCBI) 10412
RRIDAB_2881752
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO95478
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TINP1 (TGF beta inducible nuclear protein 1) was widely expressed in various cell lines. TINP1 overexpression significantly promoted tumor cell proliferation, which may be associated with the downregulation of p53 expression. Furthermore, TINP1 promoted a number of cell lines to the S phase of the cell cycle. TINP1 promotes cell proliferation and significantly reduces p53 and p21 expression.

实验方案

Product Specific Protocols
WB protocol for TINP1 antibody 66374-1-IgDownload protocol
IHC protocol for TINP1 antibody 66374-1-IgDownload protocol
IF protocol for TINP1 antibody 66374-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols