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TINP1 Monoclonal antibody, PBS Only

TINP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E3B11

Cat No : 66374-1-PBS

Print datasheet

Synonyms

CDK105, Hairy cell leukemia protein 1, HCL G1, HUSSY29, NSA2, TINP1, YR 29

验证数据展示

  • WB analysis of L02 using 66374-1-Ig (same clone as 66374-1-PBS)

    WB analysis of L02 using 66374-1-Ig (same clone as 66374-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66374-1-Ig (TINP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66374-1-Ig (same clone as 66374-1-PBS)

    IHC staining of human breast cancer using 66374-1-Ig (same clone as 66374-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66374-1-Ig (TINP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66374-1-Ig (same clone as 66374-1-PBS)

    IHC staining of human breast cancer using 66374-1-Ig (same clone as 66374-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66374-1-Ig (TINP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 66374-1-Ig (same clone as 66374-1-PBS)

    IF Staining of HEK-293 using 66374-1-Ig (same clone as 66374-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TINP1 antibody (66374-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 66374-1-Ig (same clone as 66374-1-PBS)

    IF Staining of HEK-293 using 66374-1-Ig (same clone as 66374-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TINP1 antibody (66374-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66374-1-PBS in a different storage buffer formulation.

产品信息

66374-1-PBS targets TINP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen TINP1 fusion protein Ag8822 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TGF beta-inducible nuclear protein 1
Synonyms CDK105, Hairy cell leukemia protein 1, HCL G1, HUSSY29, NSA2, TINP1, YR 29
Calculated Molecular Weight 260 aa, 30 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC005288
Gene Symbol TINP1
Gene ID (NCBI) 10412
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO95478
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TINP1 (TGF beta inducible nuclear protein 1) was widely expressed in various cell lines. TINP1 overexpression significantly promoted tumor cell proliferation, which may be associated with the downregulation of p53 expression. Furthermore, TINP1 promoted a number of cell lines to the S phase of the cell cycle. TINP1 promotes cell proliferation and significantly reduces p53 and p21 expression.