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TLE3 Monoclonal antibody

TLE3 Monoclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D11A8

Cat no : 66083-1-Ig

Print datasheet

Synonyms

ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3

验证数据展示

  • WB analysis of HEK-293 using 66083-1-Ig

    WB analysis of HEK-293 using 66083-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66083-1-Ig (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 66083-1-Ig

    IHC staining of human breast cancer using 66083-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66083-1-Ig (TLE3 Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with TLE3 antibody included
  • IP experiment of HEK-293 using 66083-1-Ig

    IP experiment of HEK-293 using 66083-1-Ig

    IP result of anti-TLE3 (IP:66083-1-Ig, 5ug; Detection:66083-1-Ig 1:500) with HEK-293 cells lysate 1480ug.

经过测试的应用

Positive WB detected inHEK-293 cells, human brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TLE3 Ready-To-Use IHC Kit. TLE3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66083-1-Ig targets TLE3 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human
Immunogen TLE3 fusion protein Ag17906 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
Synonyms ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3
Calculated Molecular Weight 772 aa, 83 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC015729
Gene Symbol TLE3
Gene ID (NCBI) 7090
RRIDAB_11182267
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ04726
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane therapy.(PMID:19309506 )

实验方案

Product Specific Protocols
WB protocol for TLE3 antibody 66083-1-IgDownload protocol
IHC protocol for TLE3 antibody 66083-1-IgDownload protocol
IP protocol for TLE3 antibody 66083-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols