• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HEK-293 using 66083-1-Ig

    WB analysis of HEK-293 using 66083-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66083-1-Ig (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 66083-1-Ig

    IHC staining of human breast cancer using 66083-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66083-1-Ig (TLE3 Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with TLE3 antibody included
  • IP experiment of HEK-293 using 66083-1-Ig

    IP experiment of HEK-293 using 66083-1-Ig

    IP result of anti-TLE3 (IP:66083-1-Ig, 5ug; Detection:66083-1-Ig 1:500) with HEK-293 cells lysate 1480ug.

TLE3 Monoclonal antibody

TLE3 Monoclonal Antibody for WB, IP, IHC, ELISA
Cat No. 66083-1-Ig

产品说明书

CloneNo. 1D11A8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IP, IHC, ELISA

别名

ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293 cells, human brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TLE3 Ready-To-Use IHC Kit. TLE3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66083-1-Ig targets TLE3 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human
免疫原 TLE3 fusion protein Ag17906 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
别名 ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3
计算分子量 772 aa, 83 kDa
观测分子量 90 kDa
GenBank蛋白编号BC015729
基因名称 TLE3
Gene ID (NCBI) 7090
RRIDAB_11182267
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ04726
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane therapy.(PMID:19309506 )

实验方案

Product Specific Protocols
WB protocol for TLE3 antibody 66083-1-IgDownload protocol
IHC protocol for TLE3 antibody 66083-1-IgDownload protocol
IP protocol for TLE3 antibody 66083-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols