验证数据展示
经过测试的应用
Positive IF-P detected in | human tonsillitis tissue |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67317 targets TLR8 in IF-P applications and shows reactivity with Human samples.
Tested Applications | IF-P Application Description |
Tested Reactivity | Human |
Immunogen | TLR8 fusion protein Ag16566 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | toll-like receptor 8 |
Synonyms | CD288, TLR8, toll like receptor 8 |
Calculated Molecular Weight | 1041 aa, 120 kDa |
Observed Molecular Weight | 120 kDa |
GenBank Accession Number | BC101075 |
Gene Symbol | TLR8 |
Gene ID (NCBI) | 51311 |
RRID | AB_2919451 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q9NR97 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Toll-like receptors (TLRs) are a group of pathogen recognition receptors (PRRs) that can be divided into two subfamilies depending on their subcellular localization(PMID: 32268138). Toll-like receptor 8 (TLR8), a sensor for pathogen-derived single-stranded RNA (ssRNA), binds to uridine (Uri) and ssRNA to induce defense responses (PMID:30535046). TLR8 proteins were expressed in spleen, epididymal sperm, testicular sperm cells, and Leydig cells but were weak in Sertoli cells. It can identify two isoforms,120 and 122 kDa.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 TLR8 antibody CL488-67317 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |