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验证数据展示

  • IF Staining of mouse brain using CL488-20718

    IF Staining of mouse brain using CL488-20718

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 TMC1 antibody (CL488-20718) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 488-conjugated TMC1 Polyclonal antibody

TMC1 Polyclonal Antibody for IF-P
Cat No. CL488-20718

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P

别名

DFNA36, DFNB11, DFNB7, TMC1, transmembrane channel like 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 20718-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-20718 targets TMC1 in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane channel-like 1
别名 DFNA36, DFNB11, DFNB7, TMC1, transmembrane channel like 1
计算分子量 88 kDa
观测分子量 88 kDa
GenBank蛋白编号NM_138691
基因名称 TMC1
Gene ID (NCBI) 117531
RRIDAB_3672717
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TDI8
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transmembrane channel-like protein 1 (TMC1), is an integral membrane protein with six transmembrane domains and cytoplasmically oriented N- and C- termini (PMID: 20672865). Human TMC1 and mouse Tmc1 mRNA expression were detected in human fetal cochlea and inner-ear tissue of postnatal mouse, respectively. TMC1 is required for cochlear hair-cell function (PMID: 11850618). It is a component of hair cell transduction channels and contributes to permeation properties (PMID: 23871232). Mutations in TMC1 gene can cause progressive postlingual hearing loss and profound prelingual deafness (PMID: 11850618). The antibody is specific to TMC1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TMC1 antibody CL488-20718Download protocol
Standard Protocols
Click here to view our Standard Protocols