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验证数据展示

  • FC experiment of HeLa using CL594-67876

    FC experiment of HeLa using CL594-67876

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TMP21 (CL594-67876, Clone:3B11F9) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated TMP21 Monoclonal antibody

TMP21 Monoclonal Antibody for FC (Intra)
Cat No. CL594-67876

产品说明书

CloneNo. 3B11F9

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig

应用

FC (Intra)

别名

TMED10, Protein TMED10, p24delta1, p24 family protein delta-1, p23

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67876-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67876 targets TMP21 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 TMP21 fusion protein Ag7516 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 transmembrane emp24-like trafficking protein 10 (yeast)
别名 TMED10, Protein TMED10, p24delta1, p24 family protein delta-1, p23
计算分子量 25 kDa
观测分子量21 kDa
GenBank蛋白编号BC001825
基因名称 TMED10
Gene ID (NCBI) 10972
RRIDAB_2923929
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49755
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL594 TMP21 antibody CL594-67876Download protocol
Standard Protocols
Click here to view our Standard Protocols