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TMEM100 Polyclonal antibody

TMEM100 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 25581-1-AP

Print datasheet

Synonyms

Transmembrane protein 100

验证数据展示

  • IHC staining of human lung using 25581-1-AP

    IHC staining of human lung using 25581-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung using 25581-1-AP

    IHC staining of human lung using 25581-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 25581-1-AP

    IHC staining of mouse brain using 25581-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25581-1-AP (TMEM100 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inmouse brain tissue, human brain tissue, human lung tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25581-1-AP targets TMEM100 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen TMEM100 fusion protein Ag22272 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 100
Synonyms Transmembrane protein 100
Calculated Molecular Weight 134 aa, 14 kDa
GenBank Accession NumberBC010128
Gene Symbol TMEM100
Gene ID (NCBI) 55273
RRIDAB_2880138
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NV29
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for TMEM100 antibody 25581-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Biomolecules

Identification of a Fibroblast-Related Prognostic Model in Glioma Based on Bioinformatics Methods

Authors - Haofuzi Zhang
View Article
humanWB

World J Clin Oncol

Regulation of TMEM100 expression by epigenetic modification, effects on proliferation and invasion of esophageal squamous carcinoma

Authors - Yue-Feng Xu
View Article
humanIF

Front Cell Neurosci

A novel histone deacetylase inhibitor W2A-16 improves the barrier integrity in brain vascular endothelial cells

Authors - Yasuteru Inoue
View Article