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TMEM111 Monoclonal antibody

TMEM111 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67205-1-Ig

产品说明书

CloneNo. 2F8G5

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (2)

EMC3, POB, TMEM111, transmembrane protein 111

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, human heart tissue, pig heart tissue, rat heart tissue, mouse heart tissue, HepG2 cells, Jurkat cells, HSC-T6, NIH/3T3 cells, 4T1 cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67205-1-Ig targets TMEM111 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IP, IF
经测试反应性 Human, mouse, rat, pig
文献引用反应性human
免疫原 TMEM111 fusion protein Ag18365 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 transmembrane protein 111
别名 EMC3, POB, TMEM111, transmembrane protein 111
计算分子量 261 aa, 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC022807
基因名称 TMEM111
Gene ID (NCBI) 55831
RRIDAB_2882498
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9P0I2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM111,also names as EMC3, is part of the endoplasmic reticulum-associated secretory pathway (PMID: 19797678). TMEM111 was required for murine pulmonary surfactant synthesis and lung function at birth (PMID: 29083321). It can be detect a band of 30 kDa.

实验方案

Product Specific Protocols
WB protocol for TMEM111 antibody 67205-1-IgDownload protocol
IHC protocol for TMEM111 antibody 67205-1-IgDownload protocol
IF protocol for TMEM111 antibody 67205-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IP

Mol Cell

WNK1 is an assembly factor for the human ER membrane protein complex.

Authors - Tino Pleiner
humanWB

J Cell Biol

A selectivity filter in the ER membrane protein complex limits protein misinsertion at the ER

Authors - Tino Pleiner
humanWB

bioRxiv

Role of a holo-insertase complex in the biogenesis of biophysically diverse ER membrane proteins

Authors - Katharine R Page
humanIF

EMBO J

The ER membrane protein complex restricts mitophagy by controlling BNIP3 turnover

Authors - Jose M Delgado
  • KO Validated
humanWB

Science

MTCH2 is a mitochondrial outer membrane protein insertase

Authors - Alina Guna
humanIF

bioRxiv

The ER membrane protein complex governs lysosomal turnover of a mitochondrial tail-anchored protein, BNIP3, to restrict mitophagy

Authors - Jose M Delgado
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