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  • Featured Product
  • KD/KO Validated

TMEM111 Monoclonal antibody, PBS Only

TMEM111 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F8G5

Cat no : 67205-1-PBS

Print datasheet

Synonyms

EMC3, POB, TMEM111, transmembrane protein 111

验证数据展示

  • WB analysis using 67205-1-Ig (same clone as 67205-1-PBS)

    WB analysis using 67205-1-Ig (same clone as 67205-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67205-1-Ig (TMEM111 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 67205-1-Ig (same clone as 67205-1-PBS)

    IHC staining of mouse lung using 67205-1-Ig (same clone as 67205-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67205-1-Ig (TMEM111 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 67205-1-Ig (same clone as 67205-1-PBS)

    IHC staining of mouse lung using 67205-1-Ig (same clone as 67205-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67205-1-Ig (TMEM111 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67205-1-Ig (same clone as 67205-1-PBS)

    WB analysis of HEK-293 using 67205-1-Ig (same clone as 67205-1-PBS)

    WB result of TMEM111 antibody (67205-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM111 transfected HEK-293 cells. This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 67205-1-Ig (same clone as 67205-1-PBS)

    IF Staining of A431 using 67205-1-Ig (same clone as 67205-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using TMEM111 antibody (67205-1-Ig, Clone: 2F8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

产品信息

67205-1-PBS targets TMEM111 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen TMEM111 fusion protein Ag18365 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name transmembrane protein 111
Synonyms EMC3, POB, TMEM111, transmembrane protein 111
Calculated Molecular Weight 261 aa, 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC022807
Gene Symbol TMEM111
Gene ID (NCBI) 55831
RRIDAB_2882498
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P0I2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMEM111,also names as EMC3, is part of the endoplasmic reticulum-associated secretory pathway (PMID: 19797678). TMEM111 was required for murine pulmonary surfactant synthesis and lung function at birth (PMID: 29083321). It can be detect a band of 30 kDa.