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TMEM160 Polyclonal antibody

TMEM160 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26451-1-AP

Print datasheet

Synonyms

Transmembrane protein 160

验证数据展示

  • WB analysis using 26451-1-AP

    WB analysis using 26451-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26451-1-AP (TMEM160 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 26451-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 26451-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26451-1-AP (TMEM160 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 26451-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 26451-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26451-1-AP (TMEM160 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26451-1-AP targets TMEM160 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen TMEM160 fusion protein Ag24915 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 160
Synonyms Transmembrane protein 160
Calculated Molecular Weight 188 aa, 20 kDa
Observed Molecular Weight15-20 kDa
GenBank Accession NumberBC002907
Gene Symbol TMEM160
Gene ID (NCBI) 54958
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NX00
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The transmembrane protein (TMEM) family encompasses transmembrane proteins that traverse the lipid bilayer and wield critical functions across various cell types, encompassing inflammation and store-operated Ca2+ entry. TMEM160, a identified transmembrane protein that is localized in the mitochondrial membrane, has been implicated in neuropathic pain.

实验方案

Product Specific Protocols
WB protocol for TMEM160 antibody 26451-1-APDownload protocol
IHC protocol for TMEM160 antibody 26451-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols