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TMEM168 Polyclonal antibody

TMEM168 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 22527-1-AP

Print datasheet

Synonyms

TMEM168, transmembrane protein 168

验证数据展示

  • WB analysis using 22527-1-AP

    WB analysis using 22527-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22527-1-AP (TMEM168 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 22527-1-AP

    IHC staining of human testis using 22527-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22527-1-AP (TMEM168 Antibody) at dilution of 1:50.

  • IHC staining of human testis using 22527-1-AP

    IHC staining of human testis using 22527-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22527-1-AP (TMEM168 Antibody) at dilution of 1:50.

经过测试的应用

Positive WB detected inmouse brain tissue, PC-3 cells, rat brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22527-1-AP targets TMEM168 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TMEM168 fusion protein Ag18337 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 168
Synonyms TMEM168, transmembrane protein 168
Calculated Molecular Weight 697 aa, 80 kDa
Observed Molecular Weight 110-130 kDa
GenBank Accession NumberBC030804
Gene Symbol TMEM168
Gene ID (NCBI) 64418
RRIDAB_2879118
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9H0V1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TMEM168 antibody 22527-1-APDownload protocol
IHC protocol for TMEM168 antibody 22527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

FASEB J

Identification of transmembrane protein 168 mutation in familial Brugada syndrome.

Authors - Akio Shimizu
View Article
humanIP, WB

J Biochem

Transmembrane protein 168 mutation reduces cardiomyocyte cell surface expression of Nav1.5 through αB-crystallin intracellular dynamics.

Authors - Le Kim Chi Nguyen
View Article

Oncol Res

Inhibition of proliferation by knockdown of transmembrane (TMEM) 168 in glioblastoma cells via suppression of Wnt/beta-catenin pathway.

Authors - Jie Xu
View Article