验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, PC-3 cells, rat brain tissue |
Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
22527-1-AP targets TMEM168 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IP, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human |
免疫原 | TMEM168 fusion protein Ag18337 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | transmembrane protein 168 |
别名 | TMEM168, transmembrane protein 168 |
计算分子量 | 697 aa, 80 kDa |
观测分子量 | 110-130 kDa |
GenBank蛋白编号 | BC030804 |
基因名称 | TMEM168 |
Gene ID (NCBI) | 64418 |
RRID | AB_2879118 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen Affinity purified |
UNIPROT ID | Q9H0V1 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for TMEM168 antibody 22527-1-AP | Download protocol |
IHC protocol for TMEM168 antibody 22527-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
FASEB J Identification of transmembrane protein 168 mutation in familial Brugada syndrome. | ||
J Biochem Transmembrane protein 168 mutation reduces cardiomyocyte cell surface expression of Nav1.5 through αB-crystallin intracellular dynamics. | ||
Oncol Res Inhibition of proliferation by knockdown of transmembrane (TMEM) 168 in glioblastoma cells via suppression of Wnt/beta-catenin pathway. |