- Featured Product
- KD/KO Validated
TMEM173/STING Monoclonal antibody
TMEM173/STING Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Rat and More (2)
Applications
WB, IHC, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
1F1E1
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, HSC-T6 cells, HEK-293 cells, THP-1 cells, MOLT-4 cells, Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy STING Ready-To-Use IHC Kit. STING primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66680-1-Ig targets TMEM173/STING in WB, IHC, IF, IP, ELISA applications and shows reactivity with Human, Rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | Human, Rat |
| Cited Reactivity | human, rat |
| Immunogen | TMEM173/STING fusion protein Ag20363 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | transmembrane protein 173 |
| Synonyms | FLJ38577, hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, NET23, STING, TMEM173, TMEM173/STING, transmembrane protein 173 |
| Calculated Molecular Weight | 379 aa, 42 kDa |
| Observed Molecular Weight | 37 kDa |
| GenBank Accession Number | BC047779 |
| Gene Symbol | STING |
| Gene ID (NCBI) | 340061 |
| RRID | AB_2882034 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q86WV6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM173/STING antibody 66680-1-Ig | Download protocol |
| IHC protocol for TMEM173/STING antibody 66680-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity Golgi apparatus-synthesized sulfated glycosaminoglycans mediate polymerization and activation of the cGAMP sensor STING. | ||
Cell Death Differ The DJ1-Nrf2-STING axis mediates the neuroprotective effects of Withaferin A in Parkinson's disease.
| ||
Cell Death Differ Inhibition of cGAS-STING by JQ1 alleviates oxidative stress-induced retina inflammation and degeneration. | ||
Autophagy Lipotoxicity-induced STING1 activation stimulates MTORC1 and restricts hepatic lipophagy.
| ||
Oncogene GBP3 promotes glioblastoma resistance to temozolomide by enhancing DNA damage repair. | ||
Free Radic Biol Med STING promotes ferroptosis through NCOA4-dependent ferritinophagy in acute kidney injury |
