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  • Featured Product
  • KD/KO Validated

TMEM173/STING Monoclonal antibody, PBS Only

TMEM173/STING Monoclonal Antibody for WB,IHC,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat

Applications

WB,IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1F1E1

Cat No : 66680-1-PBS

Print datasheet

Synonyms

FLJ38577, hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, NET23, STING, TMEM173, TMEM173/STING, transmembrane protein 173

验证数据展示

  • WB analysis of THP-1 using 66680-1-Ig (same clone as 66680-1-PBS)

    WB analysis of THP-1 using 66680-1-Ig (same clone as 66680-1-PBS)

    WB result of TMEM173/STING antibody (66680-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected THP-1 cells. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • WB analysis using 66680-1-Ig (same clone as 66680-1-PBS)

    WB analysis using 66680-1-Ig (same clone as 66680-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    IHC staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STING antibody included
  • IHC staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    IHC staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STING antibody included
Planning an IHC experiment? We recommend our IHCeasy STING Ready-To-Use IHC Kit. STING primary antibody included.

产品信息

66680-1-PBS targets TMEM173/STING in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications WB,IHC,ELISA Application Description
Tested Reactivity Human, Rat
Immunogen TMEM173/STING fusion protein Ag20363 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name transmembrane protein 173
Synonyms FLJ38577, hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, NET23, STING, TMEM173, TMEM173/STING, transmembrane protein 173
Calculated Molecular Weight 379 aa, 42 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC047779
Gene Symbol TMEM173
Gene ID (NCBI) 340061
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86WV6
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).