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验证数据展示

  • WB analysis of THP-1 using 66680-1-Ig (same clone as 66680-1-PBS)

    WB analysis of THP-1 using 66680-1-Ig (same clone as 66680-1-PBS)

    WB result of TMEM173/STING antibody (66680-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected THP-1 cells. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • WB analysis using 66680-1-Ig (same clone as 66680-1-PBS)

    WB analysis using 66680-1-Ig (same clone as 66680-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    IHC staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STING antibody included
  • IHC staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    IHC staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STING antibody included
  • IF Staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    IF Staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using TMEM173/STING antibody (66680-1-Ig, Clone: 1F1E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TMEM173/STING Monoclonal antibody, PBS Only

TMEM173/STING Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66680-1-PBS

产品说明书

CloneNo. 1F1E1

宿主/亚型

Mouse / IgG2b

种属反应性

human, rat

应用

WB, IHC, IF-P, ELISA

别名

STING, NET23, MPYS, MITA, ERIS

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy STING Ready-To-Use IHC Kit. STING primary antibody included.

产品信息

66680-1-PBS targets TMEM173/STING in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, rat
免疫原 TMEM173/STING fusion protein Ag20363 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 transmembrane protein 173
别名 STING, NET23, MPYS, MITA, ERIS
计算分子量 379 aa, 42 kDa
观测分子量 37 kDa
GenBank蛋白编号BC047779
基因名称 STING
Gene ID (NCBI) 340061
RRIDAB_2882034
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86WV6
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).