• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

TMEM173/STING Monoclonal antibody, PBS Only

TMEM173/STING Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F1E1

Cat no : 66680-1-PBS

Print datasheet

Synonyms

FLJ38577, hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, NET23, STING, TMEM173, TMEM173/STING, transmembrane protein 173

验证数据展示

  • WB analysis of THP-1 using 66680-1-Ig (same clone as 66680-1-PBS)

    WB analysis of THP-1 using 66680-1-Ig (same clone as 66680-1-PBS)

    WB result of TMEM173/STING antibody (66680-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected THP-1 cells. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • WB analysis using 66680-1-Ig (same clone as 66680-1-PBS)

    WB analysis using 66680-1-Ig (same clone as 66680-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    IHC staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STING antibody included
  • IHC staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    IHC staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STING antibody included
Planning an IHC experiment? We recommend our IHCeasy STING Ready-To-Use IHC Kit. STING primary antibody included.

产品信息

66680-1-PBS targets TMEM173/STING in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Rat
Immunogen TMEM173/STING fusion protein Ag20363 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name transmembrane protein 173
Synonyms FLJ38577, hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, NET23, STING, TMEM173, TMEM173/STING, transmembrane protein 173
Calculated Molecular Weight 379 aa, 42 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC047779
Gene Symbol STING
Gene ID (NCBI) 340061
RRIDAB_2882034
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86WV6
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).