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验证数据展示

  • WB analysis using 19925-1-AP (same clone as 19925-1-PBS)

    WB analysis using 19925-1-AP (same clone as 19925-1-PBS)

    Boiled and unboiled HeLa cell lysates were subjected to SDS PAGE followed by western blot with 19925-1-AP (TMEM175 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19925-1-PBS in a different storage buffer formulation.

  • WB analysis using 19925-1-AP (same clone as 19925-1-PBS)

    WB analysis using 19925-1-AP (same clone as 19925-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19925-1-AP (TMEM175 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19925-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 19925-1-AP (same clone as 19925-1-PBS)

    IHC staining of mouse brain using 19925-1-AP (same clone as 19925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19925-1-AP (TMEM175 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19925-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 19925-1-AP (same clone as 19925-1-PBS)

    IHC staining of mouse brain using 19925-1-AP (same clone as 19925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19925-1-AP (TMEM175 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19925-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TMEM175 Polyclonal antibody, PBS Only

TMEM175 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 19925-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Transmembrane protein 175, hTMEM175

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

19925-1-PBS targets TMEM175 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TMEM175 fusion protein Ag13890 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 175
别名 Transmembrane protein 175, hTMEM175
计算分子量 504 aa, 56 kDa
观测分子量41-45 kDa, 54-60 kDa
GenBank蛋白编号BC005158
基因名称 TMEM175
Gene ID (NCBI) 84286
RRIDAB_10666166
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BSA9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability. It has two isoforms with MW 41-45 kDa and 54-60 kDa. For optimal WB detection with this antibody, we recommend avoiding boiling the sample after lysis.