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验证数据展示

  • WB analysis using 19925-1-AP (same clone as 19925-1-PBS)

    WB analysis using 19925-1-AP (same clone as 19925-1-PBS)

    Boiled and unboiled HeLa cell lysates were subjected to SDS PAGE followed by western blot with 19925-1-AP (TMEM175 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19925-1-PBS in a different storage buffer formulation.

  • WB analysis using 19925-1-AP (same clone as 19925-1-PBS)

    WB analysis using 19925-1-AP (same clone as 19925-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19925-1-AP (TMEM175 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19925-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 19925-1-AP (same clone as 19925-1-PBS)

    IHC staining of mouse brain using 19925-1-AP (same clone as 19925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19925-1-AP (TMEM175 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19925-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 19925-1-AP (same clone as 19925-1-PBS)

    IHC staining of mouse brain using 19925-1-AP (same clone as 19925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19925-1-AP (TMEM175 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19925-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TMEM175 Polyclonal antibody, PBS Only

TMEM175 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 19925-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Transmembrane protein 175, hTMEM175

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

19925-1-PBS targets TMEM175 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TMEM175 fusion protein Ag13890 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 175
Synonyms Transmembrane protein 175, hTMEM175
Calculated Molecular Weight 504 aa, 56 kDa
Observed Molecular Weight41-45 kDa, 54-60 kDa
GenBank Accession NumberBC005158
Gene Symbol TMEM175
Gene ID (NCBI) 84286
RRIDAB_10666166
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BSA9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability. It has two isoforms with MW 41-45 kDa and 54-60 kDa. For optimal WB detection with this antibody, we recommend avoiding boiling the sample after lysis.