• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TMEM53 Recombinant antibody, PBS Only

TMEM53 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240511C3

Cat no : 83554-3-PBS

Print datasheet

Synonyms

Transmembrane protein 53, Nuclear envelope transmembrane protein 4, NET4, 240511C3

验证数据展示

  • IF Staining of A549 using 83554-3-RR (same clone as 83554-3-PBS)

    IF Staining of A549 using 83554-3-RR (same clone as 83554-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TMEM53 antibody (83554-3-RR, Clone: 240511C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83554-3-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83554-3-RR (same clone as 83554-3-PBS)

    FC experiment of A549 using 83554-3-RR (same clone as 83554-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug TMEM53 Recombinant antibody (83554-3-RR, Clone:240511C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83554-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83554-3-RR

    Affinity and Kinetic Characterization of 83554-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83554-3-RR against Human TMEM53 were performed. The affinity constant is 44.5 nM.

产品信息

83554-3-PBS targets TMEM53 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen TMEM53 fusion protein Ag21321 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transmembrane protein 53
Synonyms Transmembrane protein 53, Nuclear envelope transmembrane protein 4, NET4, 240511C3
Calculated Molecular Weight 277 aa, 32 kDa
GenBank Accession NumberBC064520
Gene Symbol TMEM53
Gene ID (NCBI) 79639
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ6P2H8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C