验证数据展示
经过测试的应用
Positive IF/ICC detected in | A549 cells |
Positive FC (Intra) detected in | A549 cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83554-3-RR targets TMEM53 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Applications | IF/ICC, FC (Intra), ELISA Application Description |
Tested Reactivity | human |
Immunogen | TMEM53 fusion protein Ag21321 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | transmembrane protein 53 |
Synonyms | Transmembrane protein 53, Nuclear envelope transmembrane protein 4, NET4, 240511C3 |
Calculated Molecular Weight | 277 aa, 32 kDa |
GenBank Accession Number | BC064520 |
Gene Symbol | TMEM53 |
Gene ID (NCBI) | 79639 |
RRID | AB_3671171 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q6P2H8 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMEM53 was initially described as a nuclear envelope transmembrane (NET) protein because of its nuclear envelope localization. Research has found that TMEM53 functionality does not rely on viral RNA binding or canonical IFN responses, and TMEM53 exerts an antiviral effect on other bat HKU2-related CoVs in a species-specific manner. Therefore, TMEM53 may be a potential therapeutic target for HKU2-related CoV infections and useful for preparing for future outbreaks of this viral species. (PMID: 37584551)
实验方案
Product Specific Protocols | |
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IF protocol for TMEM53 antibody 83554-3-RR | Download protocol |
FC protocol for TMEM53 antibody 83554-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |