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验证数据展示

  • IF Staining of A549 using 83554-3-RR

    IF Staining of A549 using 83554-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TMEM53 antibody (83554-3-RR, Clone: 240511C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • FC experiment of A549 using 83554-3-RR

    FC experiment of A549 using 83554-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug TMEM53 Recombinant antibody (83554-3-RR, Clone:240511C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83554-3-RR

    Affinity and Kinetic Characterization of 83554-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83554-3-RR against Human TMEM53 were performed. The affinity constant is 44.5 nM.

TMEM53 Recombinant antibody

TMEM53 Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83554-3-RR

产品说明书

CloneNo. 240511C3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

Transmembrane protein 53, Nuclear envelope transmembrane protein 4, NET4, 240511C3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83554-3-RR targets TMEM53 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 TMEM53 fusion protein Ag21321 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane protein 53
别名 Transmembrane protein 53, Nuclear envelope transmembrane protein 4, NET4, 240511C3
计算分子量 277 aa, 32 kDa
GenBank蛋白编号BC064520
基因名称 TMEM53
Gene ID (NCBI) 79639
RRIDAB_3671171
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ6P2H8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM53 was initially described as a nuclear envelope transmembrane (NET) protein because of its nuclear envelope localization. Research has found that TMEM53 functionality does not rely on viral RNA binding or canonical IFN responses, and TMEM53 exerts an antiviral effect on other bat HKU2-related CoVs in a species-specific manner. Therefore, TMEM53 may be a potential therapeutic target for HKU2-related CoV infections and useful for preparing for future outbreaks of this viral species. (PMID: 37584551)

实验方案

Product Specific Protocols
IF protocol for TMEM53 antibody 83554-3-RRDownload protocol
FC protocol for TMEM53 antibody 83554-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols