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验证数据展示

  • IF Staining of A549 using CL488-83554-3

    IF Staining of A549 using CL488-83554-3

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 TMEM53 antibody (CL488-83554-3, Clone: 240511C3 ) at dilution of 1:200.

  • FC experiment of A549 using CL488-83554-3

    FC experiment of A549 using CL488-83554-3

    1x10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated TMEM53 Recombinant antibody (CL488-83554-3, Clone:240511C3)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated TMEM53 Recombinant antibody

TMEM53 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-83554-3

Print datasheet

CloneNo. 240511C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

Transmembrane protein 53, Nuclear envelope transmembrane protein 4, NET4, 240511C3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 83554-3-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83554-3 targets TMEM53 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen TMEM53 fusion protein Ag21321 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transmembrane protein 53
Synonyms Transmembrane protein 53, Nuclear envelope transmembrane protein 4, NET4, 240511C3
Calculated Molecular Weight 277 aa, 32 kDa
GenBank Accession NumberBC064520
Gene Symbol TMEM53
Gene ID (NCBI) 79639
RRIDAB_3673272
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6P2H8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM53 was initially described as a nuclear envelope transmembrane (NET) protein because of its nuclear envelope localization. Research has found that TMEM53 functionality does not rely on viral RNA binding or canonical IFN responses, and TMEM53 exerts an antiviral effect on other bat HKU2-related CoVs in a species-specific manner. Therefore, TMEM53 may be a potential therapeutic target for HKU2-related CoV infections and useful for preparing for future outbreaks of this viral species. (PMID: 37584551)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TMEM53 antibody CL488-83554-3Download protocol
FC protocol for CL Plus 488 TMEM53 antibody CL488-83554-3Download protocol
Standard Protocols
Click here to view our Standard Protocols