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TMEM74 Monoclonal antibody

TMEM74 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2H1B7

Cat no : 67773-1-Ig

Print datasheet

Synonyms

FLJ30668, TMEM74, transmembrane protein 74

验证数据展示

  • WB analysis of LNCaP using 67773-1-Ig

    WB analysis of LNCaP using 67773-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67773-1-Ig (TMEM74 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inLNCaP cells, L02 cells, HEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67773-1-Ig targets TMEM74 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen TMEM74 fusion protein Ag22359 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name transmembrane protein 74
Synonyms FLJ30668, TMEM74, transmembrane protein 74
Calculated Molecular Weight 305 aa, 33 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC030710
Gene Symbol TMEM74
Gene ID (NCBI) 157753
RRIDAB_2918538
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96NL1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transmembrane protein 74 (TMEM74), plays an essential role in autophagy during cell starvation and other stress conditions. TMEM74 is localized to the lysosome and autophagosome. It has been rreported that TMEM74-induced autophagy may be associated with PI3K signal transduction. (PMID: 19029833, 18294959)

实验方案

Product Specific Protocols
WB protocol for TMEM74 antibody 67773-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols