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验证数据展示

  • FC experiment of HEK-293 using CL488-67773

    FC experiment of HEK-293 using CL488-67773

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TMEM74 (CL488-67773, Clone:2H1B7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated TMEM74 Monoclonal antibody

TMEM74 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67773

产品说明书

CloneNo. 2H1B7

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

FC (Intra)

别名

Transmembrane protein 74, NET36

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 67773-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67773 targets TMEM74 in FC (Intra) applications and shows reactivity with Human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human
免疫原 TMEM74 fusion protein Ag22359 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 transmembrane protein 74
别名 Transmembrane protein 74, NET36
计算分子量 305 aa, 33 kDa
观测分子量33 kDa
GenBank蛋白编号BC030710
基因名称 TMEM74
Gene ID (NCBI) 157753
RRIDAB_2934568
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96NL1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transmembrane protein 74 (TMEM74), plays an essential role in autophagy during cell starvation and other stress conditions. TMEM74 is localized to the lysosome and autophagosome. It has been rreported that TMEM74-induced autophagy may be associated with PI3K signal transduction. (PMID: 19029833, 18294959)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TMEM74 antibody CL488-67773Download protocol
Standard Protocols
Click here to view our Standard Protocols