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  • Featured Product
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TMF1 Monoclonal antibody

TMF1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3G12F2

Cat no : 67505-1-Ig

Print datasheet

Synonyms

TMF, TATA element modulatory factor, ARA160, Androgen receptor-associated protein of 160 kDa

验证数据展示

  • WB analysis using 67505-1-Ig

    WB analysis using 67505-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67505-1-Ig (TMF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67505-1-Ig

    WB analysis of HeLa using 67505-1-Ig

    WB result of TMF1 antibody (67505-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMF1 transfected HeLa cells.

  • IHC staining of mouse brain using 67505-1-Ig

    IHC staining of mouse brain using 67505-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67505-1-Ig (TMF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMF1 antibody included
  • IF Staining of HepG2 using 67505-1-Ig

    IF Staining of HepG2 using 67505-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TMF1 antibody (67505-1-Ig, Clone: 3G12F2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue, mouse kidney tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TMF1 Ready-To-Use IHC Kit. TMF1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67505-1-Ig targets TMF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TMF1 fusion protein Ag17666 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TATA element modulatory factor 1
Synonyms TMF, TATA element modulatory factor, ARA160, Androgen receptor-associated protein of 160 kDa
Calculated Molecular Weight 1093 aa, 123 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC126123
Gene Symbol TMF1
Gene ID (NCBI) 7110
RRIDAB_2882728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP82094
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMF1, also named as ARA160, is a potential coactivator of the androgen receptor. TMF1 mediates STAT3 degradation. TMF1 plays critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. TMF1 binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). The antibody is specific to TMF1. TMF1 can be detected as 150-160 kDa after posttranslational modification.

实验方案

Product Specific Protocols
WB protocol for TMF1 antibody 67505-1-IgDownload protocol
IHC protocol for TMF1 antibody 67505-1-IgDownload protocol
IF protocol for TMF1 antibody 67505-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols