验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy TMF1 Ready-To-Use IHC Kit. TMF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67505-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67505 targets TMF1 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | TMF1 fusion protein Ag17666 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | TATA element modulatory factor 1 |
| 别名 | ARA160, TATA element modulatory factor, TMF, TMF1 |
| 计算分子量 | 1093 aa, 123 kDa |
| 观测分子量 | 150-160 kDa |
| GenBank蛋白编号 | BC126123 |
| 基因名称 | TMF1 |
| Gene ID (NCBI) | 7110 |
| RRID | AB_2934771 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P82094 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMF1, also named as ARA160, is a potential coactivator of the androgen receptor. TMF1 mediates STAT3 degradation. TMF1 plays critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. TMF1 binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). The antibody is specific to TMF1. TMF1 can be detected as 150-160 kDa after posttranslational modification.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 TMF1 antibody CL594-67505 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |