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TMF1-Specific Polyclonal antibody, PBS Only

TMF1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19728-1-PBS

Print datasheet

Synonyms

验证数据展示

  • IHC staining of human cervical cancer using 19728-1-AP (same clone as 19728-1-PBS)

    IHC staining of human cervical cancer using 19728-1-AP (same clone as 19728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TMF1 antibody included
  • IHC staining of human cervical cancer using 19728-1-AP (same clone as 19728-1-PBS)

    IHC staining of human cervical cancer using 19728-1-AP (same clone as 19728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TMF1 antibody included
  • WB analysis using 19728-1-AP (same clone as 19728-1-PBS)

    WB analysis using 19728-1-AP (same clone as 19728-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19728-1-AP (TMF1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 19728-1-AP (same clone as 19728-1-PBS)

    IP experiment of HepG2 using 19728-1-AP (same clone as 19728-1-PBS)

    IP result of anti-TMF1-Specific (IP:19728-1-AP, 4ug; Detection:19728-1-AP 1:300) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 19728-1-AP (same clone as 19728-1-PBS)

    IF Staining of HepG2 using 19728-1-AP (same clone as 19728-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TMF1 Ready-To-Use IHC Kit. TMF1 primary antibody included.

产品信息

19728-1-PBS targets TMF1-Specific in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TATA element modulatory factor 1
Synonyms
Calculated Molecular Weight 123 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberNM_007114
Gene Symbol TMF1
Gene ID (NCBI) 7110
RRIDAB_10667009
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP82094
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMF1, also named as ARA160, is a potential coactivator of the androgen receptor. TMF1 mediates STAT3 degradation. TMF1 plays critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. TMF1 binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). The antibody is specific to TMF1. TMF1 can be detected as 150-160 kDa after posttranslational modification.